解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20916
更新日期:2009-01-01 00:00:00
abstract::Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An importan...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20919
更新日期:2008-12-01 00:00:00
abstract::Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20908
更新日期:2008-11-01 00:00:00
abstract::The purpose of the present study was to investigate the effects of prostaglandin (PG) E(2) and tumor necrosis factor (TNF) alpha on expression of vascular endothelial growth factor (VEGF) and its receptors, fms-like tyrosine kinase (Flt-1) and fetal liver kinase-1/kinase insert domain-containing receptor (Flk-1/KDR), ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20897
更新日期:2008-10-01 00:00:00
abstract::Acrosin is thought to fulfill several different roles in fertilization including that of a serine protease and in secondary zona pellucida (ZP) binding. However, acrosin's importance as a fertilization protein has been questioned. Especially since it was discovered that acrosin knockout mice are fertile. In this study...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20868
更新日期:2008-07-01 00:00:00
abstract::The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20859
更新日期:2008-07-01 00:00:00
abstract::Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20854
更新日期:2008-07-01 00:00:00
abstract::The present study aims at quantification of gonadotropin releasing hormone (GnRH) by radioimmunoassay, relative expression of its mRNA by real-time PCR accompanied by its cellular localization in the rat ovary by immunonohistochemistry (IHC) during different time points of pregnancy. To determine the involvement of en...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20834
更新日期:2008-06-01 00:00:00
abstract::In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20850
更新日期:2008-06-01 00:00:00
abstract::Production of embryos in vitro has enormous potential for research and commercial applications. Unfortunately, in vitro production of porcine embryos is extremely inefficient. Despite the characterization of distinct phenotypes, little is known about the molecular mechanisms and altered physiological processes of in v...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20844
更新日期:2008-06-01 00:00:00
abstract::Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20819
更新日期:2008-05-01 00:00:00
abstract::Testis-specific protein kinases are important because of their potential role in spermiogenesis, sperm maturation, and sperm function. In the present study, a novel serine-threonine kinase with high identity to human serine-threonine kinase 31 (STK31) was cloned from equine testis and expression of the protein was cha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20792
更新日期:2008-05-01 00:00:00
abstract::The objective of the present study was to determine whether glucocorticoid (GC) and its receptor (GC-R) are expressed in the porcine corpus luteum (CL), and whether GC influences porcine luteal hormone production. The gene expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11-HSD1), type 2 (11-HSD2), GC-R, and...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20833
更新日期:2008-05-01 00:00:00
abstract::Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20809
更新日期:2008-05-01 00:00:00
abstract::In this review, we discuss the expression, regulation, downstream mechanisms, and function of stress-induced stress enzymes in mammalian oocytes, peri-implantation embryos, and the stem cells derived from those embryos. Recent reports suggest that stress enzymes mediate developmental functions during early mammalian d...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.20787
更新日期:2008-04-01 00:00:00
abstract::Hexokinase is the first enzyme in the glycolytic pathway and utilizes ATP to convert glucose to glucose-6-phosphate (G6P). We previously identified three variant transcripts of Hk1 that are expressed specifically in spermatogenic cells, have different 5' untranslated regions, and encode a protein (HK1S, spermatogenic ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20791
更新日期:2008-04-01 00:00:00
abstract::Limited understanding of the importance of known pluripotency factors in pig embryonic stem cells (ESC) impedes the establishment and validation of porcine ESC lines. This study evaluated the expression of known mouse ESC and human ESC (hESC) pluripotency markers in in vivo inner cell mass (ICM) and in vitro-cultured ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20780
更新日期:2008-03-01 00:00:00
abstract::Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20751
更新日期:2008-03-01 00:00:00
abstract::So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20762
更新日期:2008-01-01 00:00:00
abstract::Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 pro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20765
更新日期:2008-01-01 00:00:00
abstract::There are five methyl binding domain (MBD) proteins characterized by a methyl CpG-binding domain. Four MBD proteins (MeCP2 and MBDs 1-3) are linked to transcriptional repression and one (MBD4), to DNA repair. During preimplantation development, the embryo undergoes global demethylation following fertilization and sele...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20712
更新日期:2008-01-01 00:00:00
abstract::This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20781
更新日期:2008-01-01 00:00:00
abstract::We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20731
更新日期:2007-11-01 00:00:00
abstract::In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20728
更新日期:2007-11-01 00:00:00
abstract::Gamete membrane interaction is critical to initiate the development of a new organism. The signaling pathways governing this event, however, are poorly understood. In this report, we provide the first evidence that protein kinase C activity in mouse eggs plays a crucial role in the regulation of this process. Stimulat...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20718
更新日期:2007-11-01 00:00:00
abstract::Smarca 2 (Brahma) and Smarca 4 (Brahma related gene 1, BRG1) alternatively occupy the catalytic site of SWI/SNF chromatin remodeling complexes. Mammalian embryos undergo a dramatic amount of epigenetic remodeling during cleavage development, which plays key roles in regulating both gene transcription and the developme...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20710
更新日期:2007-10-01 00:00:00
abstract::It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.20604
更新日期:2007-10-01 00:00:00
abstract::For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20705
更新日期:2007-10-01 00:00:00
abstract::The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transpo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20697
更新日期:2007-10-01 00:00:00
abstract::Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20674
更新日期:2007-09-01 00:00:00
abstract::Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20672
更新日期:2007-09-01 00:00:00
abstract::Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI)...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20612
更新日期:2007-09-01 00:00:00
abstract::In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20678
更新日期:2007-08-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20695
更新日期:2007-07-01 00:00:00
abstract::In the equine, the zona pellucida (ZP) is the major barrier to successful in vitro fertilization. Therefore the aim of our studies was to analyze species-specific features of the equine ZP in regard to structure and glycoprotein ZPB and ZPC expression sites during oocyte development and embryogenesis. The equine ZP re...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20501
更新日期:2007-07-01 00:00:00
abstract::The highly conserved Pumilio protein plays crucial roles in fertility of many organisms acting as a repressor of translation, and causing infertility when mutated. Although one of two human Pumilio homologs, PUMILIO2 is expressed mainly in the germ line, its role in mammalian germ cell development has not been reporte...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20683
更新日期:2007-06-01 00:00:00
abstract::Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20620
更新日期:2007-05-01 00:00:00
abstract::In the rat, in response to blastocyst implantation, stromal cells of the endometrium proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression. This phenomenon appears to be due to an active process involving apoptosis. As there is ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20524
更新日期:2007-03-01 00:00:00
abstract::Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20495
更新日期:2007-02-01 00:00:00
abstract::Many studies have shown a correlation between transferrin (Tf) concentration and sperm yield in several mammalian species. We have used transgenic mice expressing human Tf (hTf) to investigate if overexpression of Tf increases the efficiency of mouse spermatogenesis. We demonstrated that a 36% increase of Tf does not ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20523
更新日期:2007-02-01 00:00:00